| Place | Heat | Heat Place | Athlete | Age | Team/Hometown | Mark |
|---|
| Preliminary Results |
| 1 | 1 | 1 | Bianca Davis | 13 | Silver Springs Spartans Track | 26.39 |
| 1 | 2 | 1 | Autumn Vergeyle | 14 | Mississippi Valley Track Club | 26.39 |
| 3 | 1 | 2 | Crystal Malone | 13 | Joe Sims Milwaukee Striders | 26.45 |
| 4 | 2 | 2 | Tiersa Harrington | 12 | Silver Springs Spartans Track | 26.75 |
| 5 | 1 | 3 | Jacquelyn Bedingham | 12 | Northstar United Track Club | 26.89 |
| 6 | 2 | 3 | Michelle Young-Simmons | 14 | Track Minnesota | 26.90 |
| 7 | 1 | 4 | Mahnee Watts | 13 | Mississippi Valley Track Club | 27.08 |
| 8 | 1 | 5 | Emerald Egwim | 12 | Northstar United Track Club | 27.09 |
| 9 | 1 | 6 | Ashlee Downs | 14 | Central Iowa Excalibur Track C | 27.09 |
| 10 | 2 | 4 | Jaia Howard | 12 | Milwaukee Mustangs Track Club | 27.42 |
| 11 | 2 | 5 | Hannah Miller | 13 | Mississippi Valley Track Club | 27.48 |
| 12 | 2 | 6 | Toney Lock | 13 | Milwaukee Mustangs Track Club | 27.71 |
| 13 | 1 | 7 | Monica Mitchell | 13 | Milwaukee Mustangs Track Club | 28.01 |
| 14 | 1 | 8 | Mackenzie Brown | 14 | Track Minnesota | 28.59 |
| |
| Final Results |
| 1 | 1 | 1 | Autumn Vergeyle | 14 | Mississippi Valley Track Club | 26.20 |
| 2 | 1 | 2 | Tiersa Harrington | 12 | Silver Springs Spartans Track | 26.68 |
| 3 | 1 | 3 | Michelle Young-Simmons | 14 | Track Minnesota | 26.73 |
| 4 | 1 | 4 | Bianca Davis | 13 | Silver Springs Spartans Track | 26.87 |
| 5 | 1 | 5 | Jacquelyn Bedingham | 12 | Northstar United Track Club | 27.22 |
| 6 | 1 | 6 | Mahnee Watts | 13 | Mississippi Valley Track Club | 27.26 |
| 7 | 1 | 7 | Emerald Egwim | 12 | Northstar United Track Club | 27.29 |
